Biotechnology and its Applications,
Edition 2 Using Cells to Change the World
By W.T. Godbey

Publication Date: 12 Mar 2021

Biotechnology and its Applications: Using Cells to Change the World, Second Edition introduces students to the world of biotechnology in a way that runs deeper than a mere survey. Sections cover basic science, introduce cells, explain how they behave, what they are made of, demonstrate the biotechnological application of scientific principles in the laboratory, and present biotechnologies “in the real world.¿ Examples include recombinant proteins available to millions of patients, plants that have been engineered to produce food for people around the world, and regenerative medicine that may someday allow patients to receive organs that have been grown from their own cells.

The updated edition has been expanded with the most current information available, with new chapters on gene editing, bioremediation, vaccines and immunotherapy, and processing and manufacturing, thus resulting in a modern, robust, yet highly readable applications-oriented introduction to biotechnology.

Key Features

  • Takes an integrated approach from first principles, integrating cell biology, molecular biology, biochemistry, and health science
  • Presents side topics of interest throughout (“gee whiz¿ topics) to give students quick mental breaks while still extending their knowledge in a practical sense
  • Contains a greatly improved, robust teaching pedagogy to aid student learning
  • Features new chapter learning objectives, chapter summaries, highlighted key terms, more end-of-chapter questions, and a new glossary
About the author
By W.T. Godbey, Assistant Professor, Department of Chemical and Bimolecular Engineering, Tulane University, New Orleans, LA, USA
Table of Contents
Unit I – The Cell
1.  An introduction to biotechnology
1.1  An agreement with the student
1.2  Misconceptions about biotechnology
1.3  Biotechnology is broad and still expanding 

2.  Voyage into the cell      
2.1  Membranes
2.2  Cellular Transport

3.  Proteins      
3.1  Amino Acids
3.2  Protein Structure
3.3  The hydrophobic effect
3.4  A return to membranes

4.  Genes – The Blueprints for Proteins      
4.1 Nucleotides and nucleic acids
4.2 From genes to proteins 

5.  Cell Growth      
5.1  The eukaryotic cell cycle
5.2  Growth curves and their phases
5.3  Mathematics of the growth curve
5.4  Counting cell numbers
5.5  Counting cell mass
5.6  Scale-up

Unit II – Biotechnology in the laboratory
6.  Microbial killing      
6.1  The Gram stain
6.2  Microbial resistance to killing
6.3  Sterilization, disinfection, and sanitization
6.4  Microbial cell death

7.  Cell Culture and the eukaryotic cells used in biotechnology  
7.1  Adherent cells versus non-adherent cells 
7.2  Primary cells, cancer cells, cell lines 
7.3  Care and feeding

8.  Fluorescence      
8.1  Stokes’s experiments
8.2  Fluorophore properties
8.3  Fluorescence detection
8.4  Fluorescence resonance energy transfer 

9.  Agarose Gels   
9.1 Technical considerations
9.2  Application of agarose gels: gel shift
9.3  Application of agarose gels: DNA footprinting

10.  The polymerase chain reaction (PCR)   
10.1  Melt 
10.2  Anneal
10.3  Extend
10.4  PCR Loops
10.5  An application of traditional PCR
10.6  Traditional versus real-time PCR
10.7  Real-time PCR

11.  Genetic Engineering   
11.1  Plasmid architecture
11.2  Molecular cloning
11.3  A single plasmid is not enough
11.4  Spectrophotometry

Unit III – Biotechnology in the real world
12.  Gene delivery    
12.1  Gene delivery vehicles: an overview
12.2  Viral delivery methods 
12.3  Physical delivery methods 
12.4  Chemical delivery methods 
12.5  Preparation of nonviral gene delivery complexes
12.6  What is gene therapy, anyway?

13.  RNAi    
13.1  Co-suppression
13.2  RNA interference 
13.3  miRNA

14.  Genome editing
14.1 Targetable nucleases
14.2  Other genome manipulation tools
14.3  Delivery cargo

15. DNA fingerprinting    
15.1  Older DNA fingerprinting uses RFLPs
15.2  Newer DNA fingerprinting uses STRs
16.  Fermentation, beer, and biofuels    
16.1  Glycolysis
16.2  Fermentation
16.3  The production of beer
16.4  Fermentation to produce biofuels 

17.  Stem cells, tissue engineering, and regenerative medicine    
17.1  Stem cells 
17.2  Tissue engineering and regenerative medicine

18.  Transgenics and genetically modified organisms in agriculture  
18.1  Ice-minus bacteria 
18.2  Bt plants  
18.3  Herbicide resistance 
18.4  Tomatoes
18.5  Rice

19.  Patents and licenses    
19.1  Types of patents
19.2  Licenses
19.3  After a license is granted

Book details
ISBN: 9780128177266
Page Count: 480
Retail Price : £85.99

9780321766113; 9781284031409; 9780495112051; 9781555819361

Instructor Resources
First- or second-year undergraduate biology students taking an introductory course in biotechnology
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